More than just DNA’s lesser-known cousin, RNA plays a central role in turning genetic information into your body’s proteins. This remarkable molecule also carries the genetic instructions for many viruses, and it may have helped life get its start.
Together, RNA, short for ribonucleic acid, and DNA, short for deoxyribonucleic acid, make up the nucleic acids, one of the three or four classes of major “macromolecules” considered crucial for life. (The others are proteins and lipids. Many scientists also place carbohydrates in this group.) Macromolecules are very large molecules, often consisting of repeating subunits. RNA and DNA are made up of subunits called nucleotides.
The two nucleic acids team up to create proteins. The process of creating proteins using the genetic information in nucleic acids is so important to life that biologists call it “the central dogma” of molecular biology. The dogma, which describes the flow of genetic information in an organism, according to Oregon State University, says that DNA’s information gets written out, or “transcribed,” as RNA information, and RNA’s information gets written out, or “translated,” into protein.
“RNA in a basic way is the biomolecule that connects DNA and proteins,” Chuan He, a University of Chicago biologist who studies RNA modifications, told Live Science.
The ability of RNA and DNA to store and copy information depends on the molecules’ repeating nucleotide subunits. The nucleotides are organized in specific sequences, which can be read like letters in a word.
Each nucleotide has three major parts: a sugar molecule, a phosphate group and a cyclic compound called a nucleobase, or base. Sugars from different nucleotide units hook up via phosphate bridges to create the repeating polymer of an RNA or DNA molecule — like a necklace made of sugar beads linked together by phosphate strings.
The nucleobases attached to the sugars constitute the sequence information needed to build proteins, as described by the National Human Genome Research Institute. RNA and DNA each have a set of four bases: adenine, guanine, cytosine and thymine for DNA, with uracil swapping in for thymine in RNA. The four bases make up the molecules’ alphabets, and as such, are denoted as letters: A for adenine, G for guanine and so forth.
But RNA and DNA can do more than just encode “letter” sequences; they can also copy them. This works because the bases on one RNA or DNA string can stick to bases on another string, but only in a very specific way. Bases link up only with “complementary” partners: C to G and A to U in RNA (or A to T in the case of DNA). So, DNA serves as a template to transcribe an RNA molecule, which mirrors the DNA sequence — encoding a record of it.
A type of RNA called messenger RNA (mRNA) uses this copying function to ferry genetic data from DNA to the ribosomes, the protein-producing components of the cell, according to the University of Massachusetts. Ribosomes “read” mRNA sequences to determine the order in which protein subunits (amino acids) should join a growing protein molecule.
Two other RNA species complete the process: Transfer RNA (tRNA) brings amino acids specified by mRNA to the ribosomes, while ribosomal RNA (rRNA), which makes up the bulk of a ribosome, links the amino acids together.
RNA as an enzyme
Scientists consider RNA’s central dogma activities central to the molecule’s definition. But ideas about what RNA is and what it can do have greatly expanded since the 1980s, when biologists Sidney Altman and Thomas R. Cech discovered that RNA can operate like a protein. (The researchers won the 1989 Nobel Prize in Chemistry for their discovery.)
Proteins are key components for most chemical reactions in the body, serving as enzymes, thanks in part to the stunning variety of shapes, or conformations, these molecules can achieve. (Enzymes are proteins that facilitate and catalyze chemical reactions.) Unlike DNA, RNA can also shape-shift to an extent, and so can serve as an RNA-based enzyme, or ribozyme. RNA’s greater flexibility over DNA comes in part from the extra oxygen on RNA’s ribose sugar, which makes the molecule less stable, biologist Merlin Crossley wrote in The Conversation. The “deoxy” in deoxyribose references DNA’s 1-oxygen deficit.
According to some researchers, the most important RNA-based catalytic activity happens in the ribosome, where rRNA, a ribozyme, mediates amino acid addition to growing proteins. Other ribozymes include small nuclear RNAs (snRNAs), which splice mRNA into usable forms, and M1 RNA, one of the first known ribozymes, which similarly clips bacterial tRNA.
RNA’s regulatory zoo
In the last three decades, the number of known RNA varieties has blossomed, He said, as researchers discovered a menagerie of RNAs that do something completely different: regulate genes. “There’s a whole set of RNAs that play critical regulatory roles,” influencing which genes get expressed and at what rates, He said.
“In recent years, few areas of biology have been transformed as thoroughly as RNA molecular biology,” in large part due to the discovery of small regulatory RNAs, researchers wrote in a 2017 review published in the International Journal of Biomedical Science. Most significant are short interfering RNAs (siRNAs), microRNAs (miRNAs), and piwi-interacting RNAs (piRNAs), the authors wrote.
siRNAs and miRNAs “silence” genes by attaching to complementary sequences in mRNAs. The regulatory RNAs then activate complexes of proteins that can cut up the mRNA or block their translation, as described in a 2010 review published in the journal Current Genomics. siRNAs target invasive genetic material, like viral DNA, while miRNAs regulate an organism’s own genes, according to a 2009 review published in the journal Cell. piRNAs perform similar silencing, but operate specifically in sex cells, targeting moveable bits of genetic material called “transposable” elements that can mutate genes, according to a 2014 review published in the journal Development.
Michael Dhar (October 15, 2020). What is RNA? [Blog post]. Retrieved from https://buff.ly/2J528Hy